TB-500

Overview

TB-500 is a synthetic representation of the active region of Thymosin Beta-4 (Tbeta4$), a naturally occurring 43-amino-acid peptide that serves as a primary G-actin sequestering molecule in nearly all human and animal cells. It is recognized in molecular biology as a critical regulator of the cytoskeleton and cell motility.

In research settings, TB-500 is used to investigate the mechanisms of actin polymerization and cytoskeletal remodeling. By binding to G-actin, it influences cellular movement and structural organization, which are fundamental to tissue repair and regeneration. It is a cornerstone for studies focusing on the migration of cells to injury sites, the promotion of angiogenesis (new blood vessel formation), and the reduction of inflammatory signaling in muscle, tendons, and ligaments.

Key Research Applications

TB-500 is a widely used reference compound in regenerative medicine and veterinary/equine research:

• Cytoskeletal Dynamics: Investigating actin-binding interactions and the polymerization processes central to cellular structural organization.
• Angiogenesis Research: Modeling the growth of new blood vessels and its impact on nutrient delivery to ischemic or damaged tissues.
• Wound & Tissue Repair: Researching the acceleration of repair in soft tissue injuries, including muscle tears, tendonitis, and ligament strains.
• Cardiac Regeneration: Experimental models exploring the peptide’s role in cardiac repair and cell survival post-infarction.
• Dermatological Studies: Analysis of TB-500 in accelerating the healing of skin burns, surgical incisions, and corneal injuries.

Additional Details

• Synonyms: TB-500, Thymosin beta4, Timbetasin
• Sequence Length: 43 Amino Acids

Handling & Reconstitution

To preserve the complex 43-amino-acid chain of this synthetic peptide:

• Reconstitution: Use sterile water or a suitable buffered aqueous solution.
• Technique: The peptide is hygroscopic; handle under dry conditions. Gently swirl the vial until the powder is fully dissolved. Avoid vigorous agitation, as this can denature the peptide or lead to aggregation.
• Stability: Immediately aliquot the reconstituted solution into single-use portions to minimize repeated freeze-thaw cycles. Store at –20°C or below for long-term stability.
• Sterilization: If required for cell-based or in-vitro assays, filter sterilize (0.22 µm) prior to use.

Important Safety Information

For Research Use Only. This product is a synthetic peptide intended strictly for laboratory research and is not for human or veterinary use. While extensively used in equine research models, there is limited controlled human data available. All experimental outcomes depend on the biological model and concentration used; appropriate controls are recommended for all setups.

References

• Goldstein A.L., Hannappel E., Kleinman H.K. Thymosin β4: actin-sequestering protein moonlights to repair injured tissues. Trends in Molecular Medicine, 2005; 11(9): 421–429. PMID: 16099219. https://pubmed.ncbi.nlm.nih.gov/16099219/
• Malinda K.M., Sidhu G.S., Mani H., et al. Thymosin β4 accelerates wound healing. Journal of Investigative Dermatology, 1999; 113(3): 364–368. PMID: 10469335. https://pubmed.ncbi.nlm.nih.gov/10469335/
• Bock-Marquette I., Saxena A., White M.D., Dimaio J.M., Srivastava D. Thymosin β4 activates integrin-linked kinase and promotes cardiac cell migration, survival and cardiac repair. Nature, 2004; 432(7016): 466–472. PMID: 15565145. https://pubmed.ncbi.nlm.nih.gov/15565145/
• Smart N., Risebro C.A., Melville A.A., et al. Thymosin β4 induces adult epicardial progenitor mobilization and neovascularization. Nature, 2007; 445(7124): 177–182. PMID: 17108969. https://pubmed.ncbi.nlm.nih.gov/17108969/
• Growth Hormone Axis & Body Composition
• Peptide
• Consolidated Research Description